Entering the fixture with momentum after a 4-1 opening victory against Marseille, Beşiktaş aims to secure another three points on the road. Head coach Vincenzo Italiano emphasized his squad's determination to advance from the group stage during his pre-match press conference, noting the demanding qualification path the club navigated to reach this stage. Meanwhile, goalkeeper Alexander Nübel spoke to German outlet RTL about facing compatriot Oliver Baumann and adjusting to life in Istanbul, while teammate Milot Rashica, along with the injured Vaclav Cerny and ill Dusan Vlahovic, remain unavailable for the Black-Whites.
Historically, this marks Beşiktaş's first competitive meeting against Hoffenheim, as the Süper Lig side looks to improve its record against German opposition in European competition. Hoffenheim previously faced Turkish opposition twice during the 2017-2018 UEFA Avrupa Ligi group stage against İstanbul Başakşehir, recording a draw and a win.